Immunopharmacological activity of medicinal plants against Aristolochia bracteolate and Phallus impudicus

Abstract

Introduction: Immunological attempt was made to determine the potential of sevenimportant medicinally plants viz., Ficus benghalensis, Mangifera indica, Adhatoda vasica, Syzygium cumini, Terminalia arjuna, Ficus religiosa andAzadirachta indicaagainst protein antigen extracted from Aristolochia bracteolate (leaves) and Phallus impudicus (fungi).

Materials and Methods: Indirect ELISA was performed using crude protein as antigen extracted from Aristolochia bracteolate (leaves) and Phallus impudicus (fungi) and determined antibody production against aqueous leaves extract of seven different medicinal plants. In addition, in vitro assay was also performed using aqueous extract of active candidates in order to evaluate its inhibitory or stimulatory effect against these protein antigens.

Results: The result of these studies showed that Azadirachta indica(aqueous leaves extract) (followed by Mangifera indica, Ficus religiosa and Terminalia arjuna) showed higher antibody production and inhibition in proliferation rate (observed in lysed human whole blood) against protein antigen of Phallus impudicusand Aristolochia bracteolateeffectas compared to seven different medicinal plants.

Conclusion: Out of these results it is confirmed that these medicinal plants especially Azadirachta indica showed antifungal and anti-inflammatory effect against protein antigen of Phallus impudicusand Aristolochia bracteolate.

Keywords: Ficus benghalensis; Mangifera indica; Adhatoda vasica; Syzygium cumini; Terminalia arjuna; Ficus religiosa; Azadirachta indica; Immunosuppressive; ELISA