DEVELOPMENT OF HPTLC FINGERPRINTS OF FLAVANOIDS USING QUERCETIN AND BAICALEIN AS MARKERS IN OROXYLUM INDICUM (L.) VENT

Abstract

The study was aimed to develop the HPTLC fingerprints of Flavanoids using Quercetin and Baicalein as markers for leaf , stem and roots of the medicinally important plant Oroxylum indicum (L.) Vent collected from two different geographical regions of India viz., Western Ghats and North East. Preliminary phytochemical screening was done followed by extraction of flavanoids and then by HPTLC studies. Chloroform: methanol: formic acid (8.8:0.7:0.5) was used as mobile phase along with anisaldehyde  H₂SO₄  as derivatizing agent for the separation of  flavanoids. Quercetin and Baicalein were used as a marker compounds and were detected in all the plant parts collected from both the regions. A good seperation of flavanoids was observed at 540 nm after derivatisation. The study revealed diverse forms of flavanoids in large number in the root stem and leaf of Oroxylum indicum (L.) Vent. from both the regions. It can be concluded that HPTLC fingerprint analysis of root, stem and leaf extract of Oroxylum indicum (L.) Vent can be used as a diagnostic tool for the correct identification of the plant and it is useful as a phytochemical marker. These separated flavanoids in addition of being developed directly as drugs can also served as prototype drug molecules known as “Lead Compounds” and as pharmacological probes to help better understand biochemical and physiological mechanisms.

Key words: Oroxylum indicum (L.) Vent, flavanoids, HPTLC fingerprint