UV SPECTROPHOTOMETER: PRACTICAL CONSIDERATIONS

Abstract

This article deals with UV Spectroscopy its application (practical) and its mode. There are various modes of UV spectrophotometer namely photometric, spectrum, quantitation, kinetics, time scan, multi-component and bio-method. Photometric measures absorbance or % transmittance of a sample at arbitrary wavelengths. Spectrum Scans a wavelength range to measure the absorbance and % transmittance of a sample as a function of wavelength. Single beam energy measurement can also be performed. Data processing such as peak detection, smoothing, and mathematical calculation may be applied to the measured spectrum. Quantitation creates a calibration curve from a standard sample and quantitates an unknown sample. Kinetics Calculates enzyme activity from the time dependent change in absorbance. Time scan This function is used to measure the change in the rate of absorbance, transmittance, or energy in the fixed wavelength. Multi-component Enables samples with up to 8 constituent components to be measured and quantitated. In the Bio-method Mode, DNA Quantitation and Protein measurement are done. These modes are used for different analysis.