ANALYSIS OF PRIMARY METABOLITES FROM VERTICILLIUMLECANII AND STUDIED ITS IMMUNOLOGICAL ASPECTS OF CRUDE PROTEASE AGAINST RAPITHER-AB

Abstract

Objective- The objective of our study is to examine its primary metabolites (protein content) from dried powder of Verticilliumlecanii. In addition, immunological studies were conducted in order to analyzing the protease content against Rapither-AB using crude enzyme of Verticilliumlecanii in virally infected human whole blood samples.

Methods- For these studies, quantifying the protein content from Verticilliumlecanii using Nanodrop method. In contrast, crude protease isolated from crude enzyme against Rapither-AB in order to analyze its immunological activity against infected blood samples for estimating its proliferation and total cellular content. In addition, Elisa experiment were performed using protein of Verticilliumlecanii as coating antigen and measure its antibody profile of various medicinal plants against this protein antigen.

Results-The results of these studies showed production of protein at a very low concentration of dried powder, Verticilliumlecaniiand also showed the presence of protease content against crude enzyme of Verticilliumlecanii containing Rapither AB and claimed its antimicrobial properties at higher doses with respect to decline in proliferation assay and total cellular content in case of virally infected blood samples. In contrast, few of these medicinal plants are able to produce antibody production against this fungal strain i.e. Verticilliumlecanii.

Conclusion- Overall these studies indicate its antimicrobial properties of protease extracted from crude enzyme of Verticilliumlecanii against Rapither AB containing arteether.

Keywords: Verticilliumlecanii; protein; protease; Rapither AB; arteether