http://jbpr.in/index.php/jbpr/issue/feed Journal of Biomedical and Pharmaceutical Research 2018-10-15T16:48:32+00:00 Innovative Library editor@jbpr.in Open Journal Systems <p>&nbsp; &nbsp; &nbsp;<img src="/public/site/images/adminjbpr/nlm.jpg" width="219" height="144">&nbsp; &nbsp; &nbsp; &nbsp;<img src="/public/site/images/adminjbpr/www.jbpr_.in_.jpg">&nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;<a title="http://www.icmje.org/journals-following-the-icmje-recommendations/" href="http://www.icmje.org/journals-following-the-icmje-recommendations/" target="_blank" rel="noopener"><img src="/public/site/images/adminjbpr/ICMJE_newsitem-e1514876485808.jpg" width="233" height="146"></a></p> <p>&nbsp;</p> <p>&nbsp; &nbsp; &nbsp;&nbsp;<a title="|| Pub-Med NLM ID (URL) ||" href="https://www.ncbi.nlm.nih.gov/nlmcatalog/101671502" target="_blank" rel="alternate noopener noreferrer">|| Pub-Med NLM ID (URL) ||</a>&nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; <a title="https://journals.indexcopernicus.com/search/details?id=40734&amp;lang=en" href="https://journals.indexcopernicus.com/search/details?id=40734&amp;lang=en" target="_blank" rel="noopener">&nbsp;&nbsp;|| Index Copernicus (URL) ||&nbsp;</a> &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp; &nbsp;&nbsp;<a title="http://www.icmje.org/journals-following-the-icmje-recommendations/#J" href="http://www.icmje.org/journals-following-the-icmje-recommendations/#J" target="_blank" rel="noopener">&nbsp;||<strong>ICMJE (URL)||</strong></a></p> <p>&nbsp; &nbsp; &nbsp; &nbsp;</p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><span style="text-align: justify;">Journal of Biomedical and Pharmaceutical Research (JBPR) is an international, peer-reviewed, open access, online journal dedicated to the rapid publication of full-length original research papers, short communications, invited reviews, Case studies and editorial commentary and news, Opinions &amp; Perspectives and Book Reviews written at the invitation of the Editor in all areas of the Biomedical and Pharmaceutical Sciences.</span></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Medical || Dentistry || Biomedical Sciences&nbsp;|| Ayurveda&nbsp;|| Homeopathy&nbsp;||&nbsp;</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Anatomy, Physiology, Biochemistry, Molecular Biology, Cell biology, Genetics, Hematology, Pathology, Immunology, Microbiology, Virology, Parasitology, Surgery, Dental Sciences, Sports Physiology,&nbsp;Histopathology, Toxicology and all major disciplines of Biomedical Sciences.<br> <strong>Pharmaceutical Sciences || Allied Sciences&nbsp;</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Toxicology, Pharmaceutical and Biomedical Analysis, Clinical Research, Pharmacy Practice, Clinical and Hospital Pharmacy, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology and all major disciplines of Pharmaceutical&nbsp; Sciences.</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Articles are published as they are accepted and are freely available on the journal’s website to facilitate rapid and broad dissemination of research findings to a global audience.</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Top Reasons for publication with us</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Quick Quality Review:</strong> The journal has strong international team of editors and reviewers, Rapid Decision and Publication</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Very Low Publication Fees:</strong> Comparable journals charge a huge sum for each accepted manuscript. JPBR only charge the fees necessary to recoup cost associated with running the journal</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Other features:</strong> DIDS Assigned and Implemented the Open Review System (ORS).</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>Important Notice:</strong></span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Author can now directly send their manuscript as an email attachment to</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;">Innovative Library</span></span></p> <p style="text-align: justify;"><span style="font-family: lucida sans unicode,lucida grande,sans-serif;"><span style="font-size: 14px;"><strong>editor@jbpr.in</strong>, <strong>drpriyankagupta6@gmail.com</strong></span></span></p> <p>&nbsp;</p> http://jbpr.in/index.php/jbpr/article/view/556 Detection of Hepatitis B Genotypes in HBsAg & HBeAg Carriers 2018-10-15T16:48:29+00:00 Salman Khan salman186631@gmail.com Molly Madan salman186631@gmail.com <p><strong>Objective:</strong>- Hepatitis B is noteworthy medical issues that may include the late continuation of liver cirrhosis and hepatocellular carcinoma. The present study aimed for the detection and diffrentiation of Hepatitis B virus HBsAg inactive non-replicative carriers, HBeAg-positive inactive replicative carriers, active carriers &amp; HBeAg-negative chronic hepatitis B by Real Time PCR and their genotyping</p> <p><strong>Methods:</strong> This research conducted on 245 positive for HBsAg, 118 (48.16 %) were male and 127 (51.84%) were female patients, which was performed in central research station labortory of Microbiology at netaji subhash Chandra Bose subharti Medical College and Hospital, Meerut Between march 2016 to November 2017 The sera were separated and screened for HBsAg by ELISA kit. Positive samples for HBsAg were tested for HBeAg ELISA kit and DNA Viral load then sequenced for genotying</p> <p><strong>Results:</strong>. Of the 245 HBsAg Positive case 55 (1.12%) were HBeAg positive. In 16 PCR positive and HBV genotyping, In HBsAg inactive Non-Replicative 37.5% (n=6) genotype-B and 6.25% (n=1) genotype-A, In HBeAg inactive Replicative 12.5% (n=2) genotype-B and 12.5% (n=2) genotype-A and In HBeAg Active Chronic Hepatitis B 18.75% (n=3) genotype-B and 12.5% (n=2) genotype-A were detected</p> <p><strong>Conclusions:</strong>&nbsp;Management strategy, using HBsAg, HBeAg and HBV DNA viral load, seems adequate for the confirmation and diffrentiation of Hepatitis B virus inactive, active carriers &amp; HBeAg-negative chronic hepatitis B patients and genotype B was more prevalent in comparission to genotype A. Distribution of carriers &amp; genotypes, help physicians to prescribe proper antiviral/interferon therapy according to current genotyping pattern in this region</p> <p><strong>Keywords:&nbsp;</strong>Hepatitis B virus, Carrier State, HBsAg, HBeAg, RT-PCR</p> 2018-10-03T00:00:00+00:00 ##submission.copyrightStatement## http://jbpr.in/index.php/jbpr/article/view/554 ASSESSMENT OF KNOWLEDGE, ATTITUDE, AND PRACTICE OF FIRST AID, AND ASSOCIATED FACTORS, AMONG KINDERGARTEN TEACHERS 2018-10-15T16:48:32+00:00 Amsalu Birara Melese ha.birara@gmail.com Alemtsehay Alemu Tesema ha.birara@gmail.com Amare Simegn ha.birara@gmail.com <p><strong>Abstract</strong></p> <p><strong>Background:</strong> Injuries are very common now a day and can occur at any point of time in a day to day life. Among them, injuries in school children rank a major part. The objective of this research to evaluate knowledge, attitude and practice towards first aid and factors associated among kindergarten teachers.</p> <p><strong>Methods: </strong>Institutions based cross-sectional quantitative study design was employed. The study was conducted in Bahir dar town, Amhara region, Ethiopia from March to May 2017. A Pre-tested and structured questionnaire was used to collect data. Binary and multivariate logistic regression analysis was used to determine the separate and confounding effect for variables.</p> <p><strong>Result:</strong> A total of 173 respondents were interviewed with a response rate of 93%. From the total respondents 97 (56.1%) scored above mean for knowledge question, In particular, subjects lacked knowledge regarding first aid for Human/animal bite (60.1%) was not answered correctly. Regarding practice out of all respondents, 128(74%) had faced the child in need of first aid. About attitude, all respondents (100%) felt good attitude towards giving and learning first aid. Service year (AOR=8.6, 95%CI0.035-0.399) and previous first aid training (AOR=2.5, 95%CI 1.138-5.570) were found to have a significant association with knowledge of first aid.</p> <p><strong>Conclusion: </strong>The knowledge of kindergarten teachers towards first aid were found to be low while they have the good attitude towards giving first aid and receiving training on first aid. It remains necessary to increase their actual first aid knowledge and skills.</p> <p>&nbsp;</p> 2018-10-03T00:00:00+00:00 ##submission.copyrightStatement## http://jbpr.in/index.php/jbpr/article/view/557 HIGH PREVALENCE OF HUMAN PAPILLOMAVIRUS (HPV) INFECTIONS AND HIGH FREQUENCY OF MULTIPLE HPV GENOTYPES IN HUMAN IMMUNODEFICIENCY VIRUS-INFECTED WOMEN IN INDIA 2018-10-15T13:08:37+00:00 Subhash Kumar Mishra om11agra85@gmail.com Nidhi Vishnoi om11agra85@gmail.com <p>Groups of 208 human immunodeficiency virus (HIV)-infected women in India were studied for the presence of human papillomavirus with the general SPF10 PCR primer set. Virtually all (98%) women were found positive for human papillomavirus (HPV) DNA. Genotyping by the reverse hybridization line probe assay (HPV-LiPA) revealed a high prevalence of multiple genotypes (78.9% of the cases), with an average of 3.1 genotypes per patient (range, 1 to 10 genotypes). HPV 6 was the most prevalent genotype and was observed in 80 (39.2%) patients, followed by types 51 (31.9%), 11 (26.0%), 18 (24.0%), and 16 (22.5%). Of the genotypes detected, 40.9% were low-risk genotypes. Twenty-two (10.5%) patients showed normal (Pap I) cytology, 149 (71.6%) patients had inflammation (Pap II), and 28 patients (13.4%) had a Pap III score. The prevalence of high-risk genotypes increased with the cytological classification. There were no significant associations between the number of HPV genotypes detected and the cytological classification, HIV viral load, and CD4 count in these patients. In conclusion, the highly sensitive SPF10 LiPA system shows that very high proportions of HIV-infected women in Brazil is infected with HPV and often carry multiple HPV genotypes.</p> <p><strong>Key-words: </strong>HIV, Human Papillomavirus, Genotypes, Women</p> 2018-10-03T00:00:00+00:00 ##submission.copyrightStatement## http://jbpr.in/index.php/jbpr/article/view/555 GENE EXPRESSION AND SEQUENCING TECHNIQUES OF TRANSCRIPTOME TO ELUCIDATE NOVEL FUNCTION FOR PHOSPHOLIPASE (S) ENZYME 2018-10-15T16:48:31+00:00 M G Tyagi, S Saha, D Mukherjee, A Ramaswamy, PA Vora tyagi239@yahoo.co.in <p>Sequencing of RNA, or RNA-Seq, is now a common method to analyze gene expression and to uncover novel RNA species. Aspects of RNA biogenesis and metabolism can be evaluated with specialized methods for cDNA library preparation. In this study, the review examines current RNA-Seq methods for general analysis of gene expression and several specific applications, including isoform and gene fusion detection, targeted sequencing and single-cell analysis.Elucidating the transcriptome may help in better understanding of phospholipase function and novel application in diagnostics and clinical therapeutics. Therefore this review article focusses on the transcriptome analysis of the phospholipase enzymes i.e phospholipase A2, D and C isozymes and characterizing their functional roles in specialized disorders or for therapy.</p> <p><strong>Key words:</strong>&nbsp;&nbsp; RNA, sequencing, transcriptome, phospholipase D, gene, expression</p> 2018-10-03T00:00:00+00:00 ##submission.copyrightStatement##