STANDARDIZATION AND COMPARATIVE ANALYSIS OF DIFFERENT MARKETED FORMULATION OF AGNIMUKH CHURNA

18 | P a g e STANDARDIZATION AND COMPARATIVE ANALYSIS OF DIFFERENT MARKETED FORMULATION OF AGNIMUKH CHURNA Sharath M.V.*, S.M. Prasanna, G. Narayana Murthy, M.C Ravi National College of Pharmacy, Shivamogga-577201 Article Info: Received 25 December 2019; Accepted 20 January. 2020 DOI: https://doi.org/10.32553/jbpr.v9i1.713 Address for Correspondence: Sharath M.V. Conflict of interest statement: No conflict of interest ABSTRACT: The subject of herbal drug standardization is massively wide and deep, there is so much to know and so much seemingly contradictory theories on the subject of herbal medicine and its relationship with human physiology and mental function. India needs to explore the important formulations in the classical ayurvedic texts. This can be achieved only if the herbal products are evaluated and analysed using sophisticated modern techniques of standardization such as UVVisible, TLC, HPLC, HPTLC, etc. The present paper reports on Standardization and Comparative Analysis of Different Marketed Formulation of Agnimukh Churna, a poly herbal ayurvedic medicine used in treatment of anorexia, indigestion, constipation, spleen disorders, non bleeding hemorrhoids’ and abdominal pain. Agnimukh Churna was prepared as per Ayurvedic text of Yogaratnakara, India. In-house preparation and two marketed have been standardized on the basis of organoleptic characters, physical characteristics, physico-chemical properties, TLC and HPLC for the estimation of marker compound Piperine. The set parameters were found to be sufficient to evaluate the Churna and can be used as reference standards for the quality control/quality assurance laboratory of a Pharmaceutical house.


INTRODUCTION
Large number of people are acknowledging and accepting use of herbal medicines in the contemporary West. Scientific community now realizes the potential of herbs as medicines. Many factors contribute to this situation. Side and adverse effects of modern medicines is well documented. Research in basic sciences has opened up unexplored facets of natural world leading to abandoning of old concepts and enthusiastically welcoming new concepts. In light of these developments, there is a renewed interest in the plant based medicine. India can emerge as the major country and play a lead role in production of standardized, therapeutically effective Ayurvedic formulation. India needs to accept the challenge present herbal formulations in a way accepted by scientific community. This can be achieved only if the herbal products are evaluated and analysed using sophisticated modern techniques of standardization. 1 Standardization is a system to ensure that every packet of medicine that is being sold has the correct substances in correct amount and shall induce intended therapeutic effect. It is a requirement for ayurvedic formulation to undergo the quality tests in order to compete in international market so as to satisfy requirements of regulatory agencies of respective countries 2 . Therefore, an attempt has been made to standardize Agnimukh Churna; an ayurvedic formulation which is mentioned in classical ayurvedic text of Yogaratnakara, The ingredients of agnimukh Churna include Shwet Jirka (Cuiminum cyminum), Shunti (Zingibere officinale), Kale namak (Black salt) Kali marich (Piper nigrum) Nimbusatva (Citric acid), Saindavalavana (Rock salt), Pudina Satva (menthol obtained from different species of Mentha), and is used in treatment of anorexia, indigestion, constipation, spleen disorders, non bleeding hemorrhoids and abdominal pain. 3 In the present work, the Agnimukh Churna subjected to various pharmacognostical parameters. Three formulations, one in-house preparation and two samples from different manufacturers were procured and subjected to its organoleptic, physical, physico chemical analysis, TLC and chemical properties.

Methodology: 
Preparation of Agnimukh: Churna will be prepared as per the procedure given in Yogaratnakara Agirna Chikitsa.

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Powdered microscopy: It will be done for the examination of fibers, lignin, etc.

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Physico-chemical evaluation: The parameters such as extractive values, ash values and pH will be studied.

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Phytochemical investigations: To confirm the various secondary metabolites preliminary phytochemical investigation will be performed on the extracts obtained from Churna using different solvents.

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Subjecting extracts to phytochemical investigation using planar chromatographic technique.

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Compilation of data collected by in-house preparation and marketed products will be carried out.

TLC of Methanolic Extract of Agnimukh Churna for Piperine
The Alcoholic extract of all the Churnas were subjected for TLC with the standard reference Piperine. The R f values are given in the table no.18 and here the R f values of in-house and Baidyanath were 0.2625 and the Sanjevika was 0.2687 and these values were matches with the standard Piperine R f value that was 0.2550.

CONCLUSION:
The results of physical, physico chemical parameters like Loss on drying, ash value, extractive value, preliminary phytochemical screening, loss on drying, physical properties like bulk density, tap density, true density, Carr's index, Hausner's ration and analytical parameters like TLC of all the marketed formulations of Agnimukh Churna contains the intended ingredients with their standard values, there is significant difference in water soluble ash of in-house when compared to others. Rest of the values determined is having very insignificant difference.