EVALUATION OF ANTIOXIDANT ACTIVITY OF CHLOROPHYLL

Abstract

Antioxidant play an important role to protect damage caused oxidative stress (OS).Chlorophyll having phenolic contents are reported to possess antioxidant properties. The present study was designed to investigate the antioxidant properties of methanolic solution from Super Chlorophyll.

Objective: The main objective of the study was to evaluate the antioxidant activity of the chlorophyll in different validated in vitro models.

Methods: The antioxidant activities of methanolic solution were evaluated by in vitro standard method using UV-Spectrophotometer. The antioxidant activity were determined bt total antioxidant capacity, DPPH (1-1-diphennyl-2-picrylhydrzine) radical scavenging assay, Nitric Oxide scavenging assay and Hydrogen Peroxide scavenging assay methods.

Result: The solution of Chlorophyll was studied for antioxidant potential. Ascorbic acid was used as standard. This method is simple and activity of the solution is reported in term of IC₅₀ value. Antioxidant properties depend on the IC₅₀ value. Lesser the IC₅₀ value, more is the antioxidant activity. Chlorophyll showed good antioxidant activity.

Conclusion:  In case of in vitro antioxidant activity, Chlorophyll scavenged DPPH, hydrogen peroxide, nitric oxide radicals significantly, showed IC₅₀ values near to standard ascorbic acid, thus proving to have good antioxidant potential.

Keywords: Oxidative stress, DPPH, Antioxidant, Super chlorophyll.