RP-UFLC-DAD METHOD FOR THE DETERMINATION OF PIPERACILLIN AND TAZOBACTAM SODIUM IN BULK AND MARKETED FORMULATIONS

  • Kolli Anupama, B M Gurupadayya*, T Sireesha R Sairam Aditya Pharmaceutical Analysis Group, Department of Pharmaceutical Chemistry, JSS College of Pharmacy, JSS University, Mysuru – 570015, Karnataka, India

Abstract

Aim: To develop a simple, sensitive, rapid, robust and reproducible method for the simultaneous determination of piperacillin and tazobactam sodium using ultra fast chromatographic method (UFLC).

Materials and Methods: The analysis was performed on Phenomenex C18 column using acetonitrile, methanol and 1% orthophosphoric acid (pH 2.5) in the ratio of 50:30:20 v/v/v as mobile phase with flow a rate 1ml/min. The eluents were monitored with PDA detector at 225nm. The method was validated as per Q2 (R1) guidelines. The proposed optimised method is having linearity in the concentration range from 5-25 µg/ml of tazobactam sodium and 40-200µg/ml for piperacillin.

Results: In this developed method piperacillin and tazobactam sodium were eluted at a retention time of 4.5 ± 0.1min and 3.2 ± 0.1min respectively. The present method is linearity, accurate, precise and has passed all system suitability parameters.

Conclusion: The proposed method can be readily utilized for determination of piperacillin and tazobactam sodium.

Key words: Piperacillin, Tazobactam, Simultaneous determination, Validation

Published
2016-02-28
How to Cite
R Sairam Aditya, K. A. B. M. G. T. S. (2016). RP-UFLC-DAD METHOD FOR THE DETERMINATION OF PIPERACILLIN AND TAZOBACTAM SODIUM IN BULK AND MARKETED FORMULATIONS. Journal of Biomedical and Pharmaceutical Research, 5(1). Retrieved from http://jbpr.in/index.php/jbpr/article/view/101
Issue
Vol. 5 No. 1 (2016)
Section
Research Articles

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