Isolation and Identification of Keratinophilic Fungi from Soil of Gwalior Region and their Control by Methanolic Plant Extracts.

Abstract

Microorganism is ubiquition in nature. A large number of microbes are present in  our environment. The human body occurs in dynamic equilibrium with these microbes .infection occurs when a microbe penetrate the body surface of tissues.  In these it multiplies and the cumulation effect infects infections damage of disrupt tissues and organs and disease results. In the present study, we found that A. Fumigatus, T. mentagrophyte, T. rubrum. E. Floccosum and chrysosporium sp., A. Niger were the most prevalent keratinophilic fungi found in the soil of Gwalior region, which we have isolated. In vitero evalution was conducted for sensitivity testing with 5 different methanolic plant extracts for the inhibition of hyphal growth and spore formation in A. Fumigatus,, T. mentagrophyte, T. rubrum. E. Floccosum and chrysosporium sp. evalution antifungal activity was carried out by disc diffusion method and well diffusion method.

Plant secondary metabolites have been of interest to man for a lon time due to their pharmacological relevance. Higher and aeromatic plant have traditional been used in medicines due to their inhibitory effect on various microbes and they also have antifungal properties .most of their properties are due to essential oil product by their secondary metabolite.

Our study shows that fungal infection is common in human being. With the emergence of new effective system and tropical antifungal therapies. Tgere has been greater need search for alternative antifungal agent from microbes or plant.  In our study it can be concluded that keratinophilicfungi occurs in the Gwalior region and we have used methanolic plant extracts against fungi. These extracts obtained from plant material [flowers, buds, leaves, twings, bark, herbs, wood, fruits and roots]. They can also be treated against fungi. In this way we have concluded that fresh methanolic plant extracts can be used us antifungal agent as they are found be effective against the test fungi. The ultimate conclusion of this study supports the traditional medicine use of different plant extracts in treating different infections caused by pathogenic fungi in  gwalior either by using a single or combined extracts.

KEY WORDS: Isolation and identification of keratinophilic fungi from soil