COMPARISION OF LEVELS OF PERIODONTOPATHIC BACTERIA BY POLYMERASE CHAIN REACTION IN CHRONIC AND AGGRESSIVE PERIODONTITIS PATIENTS BEFORE AND AFTER NON-SURGICAL THERAPY WITH SUBGINGIVAL ANTIMICROBIAL (BIOTENE) IRRIGATION.

Abstract

INTRODUCTION: Periodontitis is an inflammatory disease of the supporting tissues of teeth caused by specific group of microorganisms resulting in destruction of   periodontal ligament, alveolar bone with pocket formation, recession or both.

AIM :  To evaluate the efficacy of  the adjunctive use of Biotene as subgingival irrigation  along with  scaling and root planning in the treatment of chronic and  aggressive periodontitis patients.

MATERIALS AND METHODS: A split mouth study was done in 40 patients having probing pocket depths of ≥4 mm and diagnosed with chronic, aggressive periodontitis.  Patients were divided into placebo group and test group. Assessment of plaque index (PI), gingival index (GI), probing pocket depth (PPD), and clinical attachment levels (CAL) was done at baseline and 3 months. Microbiologic assessment with polymerase chain reaction was done for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola by collection of plaque samples.

RESULTS:  Both the groups A and B have shown statistically significant results in terms of   all clinical and microbial parameters   pre operatively and post operatively with more significance observed.

CONCLUSION:  The results revealed that there is reduction in  all clinical parameters and microbial parameters in test groups following scaling and root planning and subgingival irrigation of Biotene when compared with SRP alone in placebo group.

Key words: Periodontitis, Biotene, Scaling and root planing, Microbial analysis.