Bioguided extraction and Evaluation of Antioxidant studies of Abutilon indicum fruits

  • Rajesh Bolleddu*1,3 , Sama Venkatesh1, Azmathunnisa Begum1, Ravi Alvala1, Rachamalla Shyamsunder2 1G. Pulla Reddy College of Pharmacy, Mehdipatnam, Hyderabad - 500 028, Telangana, India. 2Faculty of Pharmacy, University College of Chemical Technology, Osmania University, Hyderabad, Telangana. 3Vikas College of Pharmaceutical Sciences, Rayanigudem, Suryapet - 508 213, Telangana, India.

Abstract

The present study was conducted to evaluate the antioxidant activity, to determine the phytoconstituents, phenolic content and flavonoids in different fractions of Abutilon indicum fruits. The hydro alcoholic extract of Abutilon indicum fruits was fractionated with organic solvents with increasing polarity. In vitro antioxidant activity was assayed by diphenyl picryl hydrazyl radical scavenging assay, nitric oxide radical scavenging assay, superoxide radical scavenging assay, hydroxyl radical scavenging assay, ferric-reducing power assay. Phenolic content was determined by folin ciocalteu reagent method. Among all fractions the ethyl acetate fraction was found to possess good antioxidant activity, followed by chloroform fraction. The total phenolic (86 mg gallic acid equivalent/g), flavonoid (30 mg rutin equivalent/g) content in ethyl acetate fraction is high when compared to other fractions. These results reveal that ethyl acetate fraction of A. indicum fruits has strong antioxidant potential compared with all other fractions. Further studies are necessary for isolation and characterization of active constituent.

Key words: Abutilon indicum, fractions, folin ciocalteu reagent, antioxidant, flavonoid content.

Published
2016-12-30
How to Cite
Rachamalla Shyamsunder2R. B. , S. V. A. B. R. A. (2016). Bioguided extraction and Evaluation of Antioxidant studies of Abutilon indicum fruits. Journal of Biomedical and Pharmaceutical Research, 5(6). Retrieved from http://jbpr.in/index.php/jbpr/article/view/146
Issue
Vol. 5 No. 6 (2016)
Section
Research Articles

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