Demonstration the Expression of α-Methyl Acyl-Co EnzymeA Racemase, P63 and high- molecular-weight cytokeratin in Prostate biopsies in Khartoum State Hospitals

Abstract

Prostate cancer is the major health problem throughout the world. PCa remains the most common malignancy affecting men and the second leading cause of cancer-related death of men. The immunohistochemistry plays a very important role in the diagnosis, but it’s largely depends on panels of markers. Materials and Methods: This is descriptive cross sectional study carried out in Khartoum state hospitals during the period from November 2015 to September 2016. One hundred samples formalin fixed paraffin embedded biopsies were collected including 50 with BPH and 50 with adenocarcinoma of  PCa and applied by tissue micro array for IHC staining using p63, high molecular weight cytokeratin(HMWCK) and alpha methyl acyl co A racemace(AMACR) with polymer method. Results: One hundred samples were collected, out of which 43 (86%) of 50 samples benign prostatic hyperplasia and 46 (92%) of 50 samples adenocarcinoma were over the Age 60. Our result show that 33 of 50 samples had G.S 4,6,7 each of them had 11 frequencies 12 of 50 samples had G.S 5, and 5 of 50 samples with G.S 8. In our results we show that in 50 samples of BPH 7, 44 and 40 were positive in AMACR, P63 and HMWCK respectively. Also we found in 50 samples of adenocarcinoma 40, 5 and 8 were positive in AMACR, P63 and HMWCK. Conclusions: immunohistochemistry is helpful and necessary to distinguish prostate cancer from benign mimickers, and we have to use AMACR, P63 and HMWCK in combinations in routine diagnosis.

Key words: Prostate cancer, immunohistochemistry, high molecular weight cytokeratin, alpha methyl acyl co enzyme a racemace, benign prostatic hyperplasia.