IN VITRO SENSITIVITY ASSAY OF LANTANA CAMARA AGAINST AEDES AGYPTI WITH SUPPLEMENTARY FACTS FROM GC MS AND IN SILICO ANALYSIS

Abstract

In search of novel plant derived bioactive compounds, advanced methods like GC-MS and computational techniques play a crucial role to characterize and assess their effectiveness. Present study analyzed the larvicidal activity of Lantana camara against Aedes aegypti larvae to find out whether this plant can be used as a source to formulate a novel naturally derived larvicide. Larvicidal screening of petroleum ether extracts of Lantana camara showed a dose dependent growth inhibitory effect on Aedes aegypti larvae. Chemical profiling of petroleum ether extract with GC-MS showed the presence of Beta caryophyllene as a major compound and the in silico docking analysis recognized the compound as a competitive inhibitor of acetylcholine preventing its binding with acetylcholine esterase and thus nervous functioning.The results suggest that Lantana camara leaf extracts could serve as a source of compounds to formulate ecofriendly larvicides to prevent the spreading of vector-borne diseases.

Keywords: Lantana camara, Aedes aegypti, Beta caryophyllene, docking, Acetyl choline esterase