OXIDATIVE STRESS, ANTIOXIDANT STATUS AND 5’-NUCLEOTIDASE ACTIVITY IN CHRONIC HEPATITIS C AND ALCOHOLIC PATIENTS

Tarannum Fatima Subhani1, Md. Abu Nasar2, R. P Gupta2, Md. Arif Naseer3

Tarannum Fatima Subhani1, Md. Abu Nasar2, R. P Gupta2, Md. Arif Naseer3 1Department of Biochemistry, HIMS, Sitapur, India 2Department of Biochemistry, Nalanda Medical College, Patna, India 3Department of Pharmaceutical Chemistry, Adarsh Vijendra Institute of Pharmaceutical Sciences, School of Pharmacy, Shobhit University, Gangoh,

Abstract

Background: The present study was undertaken to estimate the 5’-nucleotidase enzyme in chronic hepatitis C and alcoholics. The oxidative stress, antioxidants and their correlation with 5’-nucleotidase was also included in this study.

Methods: The blood samples of 25 subjects (age and sex matched) each from group I (control), group II (alcoholic) and group III (chronic hepatitis C) was taken and centrifuged for separation of plasma for analysis of 5’-nucleotidase. The separated cells were washed thrice with 0.9 % w/v cold normal saline and used for the analysis of glutathione, malondialdehyde and superoxide dismutase.

Results: The activity of serum 5’- nucleotidase was significantly increased in both chronic hepatitis C and alcoholics. The levels of malondialdehyde were also significantly increased in both chronic hepatitis C and alcoholic patients. The levels of glutathion and superoxide dismutase were significantly decreased in both chronic hepatitis C and alcoholics.

Conclusions: From these findings it was concluded that the activity of serum 5’- nucleotidase rises consistently in chronic hepatitis C and alcoholics according to the extent of liver damage, hepatobiliary damage, and biliary stasis and can be a useful marker for diagnosis of hepatobiliay disorders.

Keywords: Alcoholics; Antioxidant effects; chronic hepatitis C; 5’- nucleotidase; Oxidative stress.