PREVALENCEOF EXTENDED SPECTRUM β LACTAMASES (ESBL) IN NON-FERMENTING GRAM NEGATIVE BACILLI FROM CLINICAL ISOLATES.

  • Thipperudraswamy .T1*, Sudhindra.K.S2, Shobha Nadigar3, Mahesh Kumar4 1Assistant Professor, Department of Microbiology, Basaveshwara Medical College & Hospital Chitradurga, Karnataka, India 2Associate Professor, Department of Microbiology, Basaveshwara Medical College & Hospital, Chitradurga, Karnataka, India 3Professor and Head, Department of Microbiology, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India 4Assocate Professor, Department of Microbiology, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India

Abstract

Background and Objectives: Non fermenting gram negative bacilli (NFGNB) producing Extended spectrum β lactamases (ESBL) are an increasing cause of concern in the hospitals as they produce a therapeutic dilemma for the treating physician. The present study was undertaken to know the prevalence of ESBL producing non fermenting gram negative bacilli from clinical isolates and their antibiotic resistance pattern. Methods: A total of 389 non fermenting gram negative bacilli were recovered from various clinical specimens. All the samples were processed for routine bacterial culture and antimicrobial susceptibility test as per standard protocol.  They were further subjected to ESBL production detection by phenotypic confirmatory double disk diffusion test using ceftazidime with and without clavulanic acid. Results: A total of 199(51.15%) isolates were found to be Extended spectrum β-lactamase producers. Majority of Acinetobactercalcoaciticus-baumaniicomplex 80 (80.8%) were ESBL producer where as 114(41.6%) of Pseudomonas aeruginosaisolates were found to be ESBLproducers. ESBL producing organisms were more drug resistant compared to ESBL non producers; Conclusion: The prevalence of ESBL was 51.15% among NFGNB. Significantly higher resistance rate was observed by these isolates to almost all the drugs routinely used.

Key words: NFGNB,Extended spectrum β-lactamase, double disk diffusion test. Pseudomonas, Acinetobacter

Published
2014-04-29
How to Cite
Shobha Nadigar3, Mahesh Kumar4T. .T1*, S. (2014). PREVALENCEOF EXTENDED SPECTRUM β LACTAMASES (ESBL) IN NON-FERMENTING GRAM NEGATIVE BACILLI FROM CLINICAL ISOLATES. Journal of Biomedical and Pharmaceutical Research, 3(2). Retrieved from http://jbpr.in/index.php/jbpr/article/view/263
Issue
Vol. 3 No. 2 (2014)
Section
Research Articles

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