DETECTION OF EXTENDED SPECTRUM BETA - LACTAMASE PRODUCING E. COLI & KLEBSIELLA SPECIES CAUSING URINARY TRACT INFECTION

Abstract

Extended-spectrum beta-lactamases (ESBLs) constitute a growing class of plasmid-mediated ß-lactamases which confer resistance to broad spectrum beta-lactam antibiotics. The frequency of ESBL producing strains among clinical isolates has been steadily increasing over the past few years resulting in limitation of the therapeutic options. This study was done to determine the susceptibility pattern of different antibiotics to ESBL producing Escherichia coli and Klebsiella spp. isolated from urine samples. Out of 70 urine samples, 62 samples showed bacterial growth. E.coli (61.29%) found to be most common bacteria in urinary tract infection followed by Klebsiella spp. (45.16%). Antimicrobial susceptibility testing was done by Kirby Bauer’s disc diffusion method. Isolates were highly resistance to Gentamicin followed by Tetracyclin, Ampicillin and Amikacin. Gentamicin showed resistance against E.coli and Klebsiella spp were 89.47% and 85.71% respectively. While Tetracyclin and Ampicillin showed 80% and 78% resistance to E.coli and Klebsiella spp. The isolates were highly susceptible against imipenem and least susceptible to Ciprofloxacin and Norfloxacin against E.coli and Klebsiella spp. These antibiotics are considered as appropriate antimicrobials for empirical treatment of urinary tract infections. Most of the ESBL producing isolates were multidrug resistant. Continuous monitoring of ESBL production and antimicrobial susceptibility testing are necessary for the treatment of UTI.

KEYWORDS: ESBL, Antibacterial susceptibility pattern, Urinary Tract infection.