A Novel Gradient RP-HPLC Method Development for Bortezomib in Parenteral Dosage Form

  • *Akshay Kumar Bisht1, Brij Bhushan2, Vandana Dhiman2, R. K. Dhawan3, U.S. Baghel3, Anil Kumar Gupta4 1Jaipur Nation University, Jaipur, Rajasthan, India-302025 2ASBASJSM College of Pharmacy, BELA, Ropar, Punjab, India-140111 3Khalsa College of Pharmacy, G.T. Road, Amritsar, Punjab, India-143002 4Research Scholar, Bhagwant University, Institute of Pharmacy & Research Center, Ajmer, Rajasthan, India-305004

Abstract

The objective of present study was the development of gradient RP-HPLC method for the estimation of bortezomib in parenteral dosage form. The analysis was performed on Hypersil C18 column (250 x 4.6mm) with mobile phase composed of acetonitrile: water: THF (65:35:5), maintaining flow rate at 1ml/min. with retention time of 3.5±0.5min. The detection was done at 280nm. A regression coefficient was 0.999 obeying the Beer’s law at concentration range of 20 – 120 µg/ml. The proposed method was validated for specificity, linearity, accuracy, precision and robustness as per ICH guidelines and used successfully for bortezomib determination in routinely quality control.

 

KEY WORDS: Validation, RP-HPLC, Bortezomib.

Published
2013-04-29
How to Cite
U.S. Baghel3, Anil Kumar Gupta4 *Akshay K. B. B. B. V. D. R. K. D. (2013). A Novel Gradient RP-HPLC Method Development for Bortezomib in Parenteral Dosage Form. Journal of Biomedical and Pharmaceutical Research, 2(2). Retrieved from http://jbpr.in/index.php/jbpr/article/view/409
Issue
Vol. 2 No. 2 (2013)
Section
Research Articles

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