Molecular Detection of HSV type 2 Infection among Infertile Males in Khartoum State, Sudan

  • Mai Elhadi Department of Medical Microbiology, Faculty of Medical Laboratory Science, El Neelain University, Khartoum, Sudan
  • Abdel Rahim M El Hussein Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan
  • Mohamed O Mustafa Department of Medical Microbiology, Faculty of Medical Laboratory Science, El Neelain University, Khartoum, Sudan
  • Isam M Elkhidir Department of Microbiology and Parasitology, Faculty of Medicine, University of Khartoum, Sudan
  • Khalid A Enan Department of Virology, Central Laboratory, Ministry of Higher Education and Scientific Research, Khartoum, Sudan
DOI: https://doi.org/10.32553/jbpr.v10i5.878
Keywords: HSV-2, RT-PCR, infertility, men, Sudan

Abstract

Background:  Herpes simplex virus (HSV) infections of humans were first documented in ancient Greece.  HSVs belong to family Herpesviridae, and are characterized by being enveloped, double-stranded DNA viruses with relatively large complex genomes.  Herpes simplex virus type 2 (HSV-2) DNA seems to play a significant role in male infertility, it is significantly related to low sperm count as well as poor motility.

Aim: This study aimed to determine the incidence of HSV-2 DNA among infertile males in Khartoum State, Sudan by using molecular methods.

Methods: The study was carried out in Khartoum State, Sudan, during May-August 2018. A total of 50 semen samples from infertile males were included. HSV-2 was detected using real-time polymerase chain reaction (RT-PCR).

Result: Among 50 semen samples from infertile males, 6(12%) samples were found positive for HSV-2 DNA.

Conclusion: Our findings showed no association between HSV-2 DNA and low sperm count in infertile Sudanese men. Our study also revealed the need for further investigations in different parts of the country to highlight the extent of the viral related men infertility problem in Sudan.

Keywords: HSV-2, RT-PCR, infertility, men, Sudan

Published
2021-10-11
How to Cite
Elhadi, M., Hussein, A. R. M. E., Mustafa, M. O., Elkhidir, I. M., & Enan, K. A. (2021). Molecular Detection of HSV type 2 Infection among Infertile Males in Khartoum State, Sudan. Journal of Biomedical and Pharmaceutical Research, 10(5). https://doi.org/10.32553/jbpr.v10i5.878
Issue
Vol. 10 No. 5 (2021)
Section
Articles

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