A Novel, Economical RP-HPLC Method for Quantification of Cefixime in Bulk and Pharmaceutical Solid Dosage form

Abstract

A new, precise, economical, and linear gradient reverse phase high-performance liquid chromatography (RP-HPLC) method has been developed and validated for assay  determination in Cefixime. The chromatographic separation was achieved with Hypersil C18(250*4.6)mmand 3 µm particle size column. The flow rate was 1.0 mL/min and eluents were detected at 220 nm using PDA detector. The retention time was found to be 7.3  min.The percentage recoveries for molecules were found to be in the range of 99–101%. The calibration curve demonstrated good linearity in the range of 20–100 gm/ml. for Cefixime. The approach has been validated in accordance with the International Conference on Harmonization's regulatory criteria. The evaluated parameters are precision, linearity, detection limit, quantification limit, specificity, accuracy, and robustness.The technique may be applied to stability investigations as well as routine analysis to identify and quantify Cefixime in pharmaceutical dosage form .

Keywords: Cefixim. Reversed Phase High Performance Liquid Chromatography. Assay substances. Methanol