Method Development and Validation of Montelukast in Bulk and Pharmaceutical Dosage form by RP-HPLC

  • Minaketan Sahoo*1, B V V Ravi Kumar2, N K Tripathy3, Saroj Kumar Patro1 1Institute of Pharmacy and Technology, Salipur, Cuttack, Odisha, India-754202 2Roland institute of pharmaceutical sciences, Berhampur, Ganjam,Odisha 3 Dept. of zoology, Berhampur University, Berhampur, Ganjam, Odisha, India

Abstract

The present work describes a simple, precise and accurate HPLC method for estimation of montelukast sodium in bulk and in tablet dosage form. Montelukast sodium is a selective and orally active leukotriene receptor antagonist that inhibits the cysteinyl leukotriene (CysLT1) receptor. The separation was achieved by using Waters symmetry shield RP-C8 column and acetonitrile: sodium di-hydrogen Phosphate dehydrate (pH 3.7) in proportion of 70:30 v/v as mobile phase, at a flow rate of 1.5 ml/min. Detection was carried out at 225 nm. The retention time of montelukast sodium was found to be 3.721 min. The limit of detection was found 0.1357 µg/ml and limit of quantification 0.4111 µg/ml. The accuracy and reliability of the proposed method was ascertained by evaluating various validation parameters like linearity (1-30 µg/ml), accuracy, precision, robustness and specificity according to ICH guidelines. The method was statistically validated and RSD was found to be less than 2% indicating high degree of accuracy and precision of the proposed HPLC method. Due to its simplicity, rapidness, high precision and accuracy, the proposed HPLC method may be used for determining Montelukast in bulk or in pharmaceutical dosage forms.

KEYWORDS: Montelukast, High Performance Liquid Chromatography, Method development, Validation

Published
2012-10-30
How to Cite
Saroj Kumar Patro1M. S. B. V. V. R. K. N. K. T. (2012). Method Development and Validation of Montelukast in Bulk and Pharmaceutical Dosage form by RP-HPLC. Journal of Biomedical and Pharmaceutical Research, 1(2). Retrieved from https://jbpr.in/index.php/jbpr/article/view/461
Section
Research Articles