ISOLATION AND CHARACTERIZATION OF A FLAVONOID FROM ETHANOLIC EXTRACT OF ALTERNANTHERA SESSILIS LINN.

Mrinmay Das*1, Ashok Kumar D2, Jyotirmoy Deb3, Durga Srinivasa Rao3

Mrinmay Das*1, Ashok Kumar D2, Jyotirmoy Deb3, Durga Srinivasa Rao3

Abstract

This study was performed to isolate and characterize the flavonoid present in Alternanthera sessilis Linn. Direct soxhlet extraction process was adopted for extraction by using 95% ethanol and vacuum evaporator was used for drying the extract. The concentrated ethanolic fraction was subjected to thin layer chromatography and column chromatography for isolation. The isolated compound was identified as a flavonoid by confirming the standard flavonoid tests: viz. Shinoda’s test. The R_f value of isolated flavonoid was calculate and different physical tests were also performed to find out its physical characteristics. Characterization of isolated flavonoid was done by FTIR, ¹H NMR and MASS. The IR spectrum indicated the presence of hydroxyl and carbonyl functions. ¹³C NMR signal indicated the presence of hydroxyl group and unsaturated keto function and four methoxy group at different position of flavones skeleton. The ¹H-NMR further showed the presence of three hydroxyl, four methoxy groups and three methine groups. On the basis of chemical and spectral analysis the structure was elucidated as 3´, 3, 6, 7-tetramethoxy - 4´, 5, 8-trihydroxy flavones.

Keywords: Flavonoids, TLC, Column Chromatography, FTIR, NMR, MASS.

Author Biography

Mrinmay Das*1, Ashok Kumar D2, Jyotirmoy Deb3, Durga Srinivasa Rao3

This study was performed to isolate and characterize the flavonoid present in Alternanthera sessilis Linn. Direct soxhlet extraction process was adopted for extraction by using 95% ethanol and vacuum evaporator was used for drying the extract. The concentrated ethanolic fraction was subjected to thin layer chromatography and column chromatography for isolation. The isolated compound was identified as a flavonoid by confirming the standard flavonoid tests: viz. Shinoda’s test. The R_f value of isolated flavonoid was calculate and different physical tests were also performed to find out its physical characteristics. Characterization of isolated flavonoid was done by FTIR, ¹H NMR and MASS. The IR spectrum indicated the presence of hydroxyl and carbonyl functions. ¹³C NMR signal indicated the presence of hydroxyl group and unsaturated keto function and four methoxy group at different position of flavones skeleton. The ¹H-NMR further showed the presence of three hydroxyl, four methoxy groups and three methine groups. On the basis of chemical and spectral analysis the structure was elucidated as 3´, 3, 6, 7-tetramethoxy - 4´, 5, 8-trihydroxy flavones.

Keywords: Flavonoids, TLC, Column Chromatography, FTIR, NMR, MASS.