IDENTIFICATION OF CLINICALLY RELEVANT MYCOBACTERIUM OTHER THAN TUBERCULOSIS (MOTT) SPECIES BY REAL-TIME PCR COUPLED WITH A HIGH-RESOLUTION MELTING SYSTEM

  • Prem Raj Singh1, Devender Singh Gill1, Preeti Caesar1, Veena Sharma*2 1Auroprobe Laboratories NH-58 Muradnagar, Ghaziabad (U.P), India. 2* Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India.

Abstract

A  real-time PCR coupled with a high-resolution melting curve assay for identifying clinically relevant MOTT species was developed and evaluated using 6  reference strains and 186 Culture isolates. Concordant results were obtained for all 186 isolates of nontuberculous mycobacteria. Our results showed that this real-time PCR -HRM assay is an effective tool for the mycobacterial identification from cultures isolates.

 

Key words: MOTT (Mycobacterium other than tuberculosis), HRM (high-resolution melting), Real time PCR.

Published
2013-12-30
How to Cite
Preeti Caesar1, Veena Sharma*2P. R. S. D. S. G. (2013). IDENTIFICATION OF CLINICALLY RELEVANT MYCOBACTERIUM OTHER THAN TUBERCULOSIS (MOTT) SPECIES BY REAL-TIME PCR COUPLED WITH A HIGH-RESOLUTION MELTING SYSTEM. Journal of Biomedical and Pharmaceutical Research, 2(6). Retrieved from http://jbpr.in/index.php/jbpr/article/view/337
Issue
Vol. 2 No. 6 (2013)
Section
Research Articles

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